Dirk Hoffmeister

Prof. Dr. Dirk Hoffmeister

Pharmazeutische Mikrobiologie · Abteilungsleiter

Telefon: +49 3641 9-49850 Telefax: +49 3641 9-49852 E-Mail: dirk.hoffmeister@leibniz-hki.de

Forschungsschwerpunkte
  • Biochemische und genetische Grundlagen des pilzlichen Sekundärstoffwechsels
  • Naturstoffe aus Basidiomyceten sowie Ralstonia solanacearum
  • Mikrobielle Peptidsynthetasen
Curriculum vitae

Wissenschaftlicher Werdegang

2014- W3-Professur Pharmazeutische Mikrobiologie, FSU Jena und HKI Jena
2009-2014 W2-Professur Pharmazeutische Biologie, FSU Jena und Leiter der Assoziierten Abteilung Pharmazeutische Mikrobiologie am HKI Jena
2007-2009 Assistant Professor (tenure track) University of Minnesota, Saint Paul, MN, USA
2008 Habilitation in Pharmazeutischer Biologie und Biotechnologie, Albert-Ludwigs-Universität Freiburg, 
2004-2007 Wiss. Assistent (C1), Institut für Pharmazie, Albert-Ludwigs-Universität Freiburg 
2002-2004 Post-Doc, University of Wisconsin, Madison, WI, USA (DFG-Stipendium)
2002 Dr. rer. nat., Chemisch-Pharmazeutische Fakultät, Albert-Ludwigs-Universität Freiburg 
1998 Diplom in Biologie an der Eberhard-Karls-Universität Tübingen

Auszeichnungen · Ämter · wissenschaftliche Aktivitäten

seit 2016 Studiendekan der Biologisch-Pharmazeutischen Fakultät der FSU Jena
2013-2016 Wiss. Beirat Vereinigung für   Allgemeine und Angewandte Mikrobiologie (VAAM).
seit 2011 Mitglied im Editorial Board verschiedener Zeitschriften
Publikationen

Baldeweg F, Kage H, Schieferdecker S, Allen C, Hoffmeister D, Nett M (2017) Structure of ralsolamycin, the inter-kingdom morphogen of the crop plant pathogen Ralstonia solanacearum GMI1000. Org Lett [Accepted] Details

Braesel J, Fricke J, Schwenk D, Hoffmeister D (2017) Biochemical and genetic basis of orsellinic acid biosynthesis and prenylation in a stereaceous basidiomycete. Fungal Genet Biol 98, 12-19. Details

Brandenburger E, Gressler M, Leonhardt R, Lackner G, Habel A, Hertweck C, Brock M, Hoffmeister D (2017) (2017) A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore. Appl Environ Microbiol [Accepted] Details

Brandt P, Garcia-Altares M, Nett M, Hertweck C, Hoffmeister D (2017) Induced chemical defense of a mushroom by a double bond-shifting polyene synthase Angew Chem Intl Ed 56, 5937-5941. Details

Brandt P, García-Altares M, Nett M, Hertweck C, Hoffmeister D (2017) Induced Chemical Defense of a Mushroom by a Double-Bond-Shifting Polyene Synthase. Angew Chem Int Ed Engl 56(21), 5937-5941. Details PubMed

Fricke J, Blei F, Hoffmeister D (2017) Enzymatic synthesis of psilocybin Angew Chem Intl Ed [Accepted] Details

Lenz C, Wick J, Hoffmeister D (2017) Identification of N-Methyl-4-hydroxytryptamine (Norpsilocin) as a Psilocybe Natural Product. J Nat Prod [Accepted] Details

Zhu Y, Mahaney J, Jellison J, Cao J, Gressler J, Hoffmeister D, Goodell B (2017) Fungal variegatic acid and extracellular polysaccharides promote the site specific generation of reactive oxygen species J Ind Microbiol Biotechnol 44, 329-338. Details PubMed

Baccile JA, Spraker JE, Le HH, Brandenburger E, Gomez C, Bok JW, Macheleidt J, Brakhage AA, Hoffmeister D, Keller NP, Schroeder FC (2016) Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus. Nat Chem Biol 12, 419-424. Details PubMed

Braga D, Hoffmeister D, Nett M (2016) A non-canonical peptide synthetase adenylates 3-methyl-2-oxovaleric acid for auriculamide biosynthesis Beilstein J Org Chem 12, 2766-2770. Details

Brandenburger E, Braga D, Kombrink A, Lackner G, Gressler J, Künzler M, Hoffmeister D (2016) Multi-genome analysis identifies functional and phylogenetic diversity of basidiomycete adenylate-forming reductases. Fungal Genet Biol [Epub ahead of print] Details PubMed

Kalb D, Gressler J, Hoffmeister D (2016) Active-Site Engineering Expands the Substrate Profile of the Basidiomycete L-Tryptophan Decarboxylase CsTDC. Chembiochem 17, 132-136. Details PubMed

Kalb D, Heinekamp T, Schieferdecker S, Nett M, Brakhage AA, Hoffmeister D (2016) An Iterative O-Methyltransferase Catalyzes 1,11-Dimethylation of Aspergillus fumigatus Fumaric Acid Amides. Chembiochem 17, 1813-1817. Details PubMed

Schlegel M, Münsterkötter M, Güldener U, Bruggmann R, Duo A, Hainaut M, Henrissat B, Sieber CM, Hoffmeister D, Grünig CR (2016) Globally distributed root endophyte Phialocephala subalpina links pathogenic and saprophytic lifestyles BMC Genomics 17, 1015. Details

Schwenk D, Brandt P, Blanchette R, Nett M, Hoffmeister D (2016) Unexpected metabolic versatility in a combined fungal fomannoxin/vibralactone biosynthesis. J. Nat. Prod. 79, 1407-1414. Details

Shah F, Nicolas C, Bentzer J, Ellström M, Smits M, Rineau F, Canbäck B, Floudas D, Carleer R, Lackner G, Braesel J, Hoffmeister D, Henrissat B, Ahrén D, Johansson T, Hibbett DS, Martin F, Persson P, Tunlid A (2016) Ectomycorrhizal fungi decompose soil organic matter using oxidative mechanisms adapted from saprotrophic ancestors. New Phytol 209, 1705-1719. Details PubMed

Shah F, Schwenk D, Nicolas C, Persson P, Hoffmeister D, Tunlid A (2016) Involutin is a Fe3+ reductant Secreted by the Ectomycorrhizal Fungus Paxillus involutus during Fenton-based Decomposition of Organic Matter. Appl Environ Microbiol 81, 8427-8433. Details PubMed

Tauber JP, Schroeckh V, Shelest E, Brakhage AA, Hoffmeister D (2016) Bacteria induce pigment formation in the basidiomycete Serpula lacrymans. Environ Microbiol 18, 5218-5227. Details PubMed

Wick J, Heine D, Lackner G, Misiek M, Tauber J, Jagusch H, Hertweck C, Hoffmeister D (2016) A fivefold parallelized biosynthetic process secures chlorination of Armillaria mellea (honey mushroom) toxins. Appl Environ Microbiol 82, 1196-1204. Details PubMed Open Access

Braesel J, Götze S, Shah F, Heine D, Tauber J, Hertweck C, Tunlid A, Stallforth P, Hoffmeister D (2015) Three redundant synthetases secure redox-active pigment production in the basidiomycete Paxillus involutus. Chem Biol 22, 1325-1334. Details PubMed

Kalb D, Heinekamp T, Lackner G, Scharf DH, Dahse H-M, Brakhage AA, Hoffmeister D (2015) Genetic engineering activates biosynthesis of aromatic fumaric acid amides in the human pathogen Aspergillus fumigatus. Appl Environ Microbiol 81, 1594-1600. Details PubMed

Kalb D, Lackner G, Rappe M, Hoffmeister D (2015) Activity of α-aminoadipate reductase depends on the N-terminally extending domain. Chembiochem 16, 1426-1430. Details PubMed

Karlsson M, Durling MB, Choi J, Kosawang C, Lackner G, Tzelepis GD, Nygren K, Dubey MK, Kamou N, Levasseur A, Zapparata A, Wang J, Amby DB, Jensen B, Sarrocco S, Panteris E, Lagopodi AL, Pöggeler S, Vannacci G, Collinge DB, Hoffmeister D, Henrissat B, Lee YH, Jensen DF (2015) Insights on the Evolution of Mycoparasitism from the Genome of Clonostachys rosea. Genome Biol Evol 7, 465-480. Details PubMed

Stadler M, Hoffmeister D (2015) Fungal natural products-the mushroom perspective. Front Microbiol 6, 127. Details PubMed

Bohnert M, Nützmann HW, Schroeckh V, Horn F, Dahse HM, Brakhage AA, Hoffmeister D (2014) Cytotoxic and antifungal activities of melleolide antibiotics follow dissimilar structure-activity relationships. Phytochemistry 105, 101-108. Details PubMed

Bohnert M, Scherer O, Wiechmann K, König S, Dahse HM, Hoffmeister D, Werz O (2014) Melleolides induce rapid cell death in human primary monocytes and cancer cells. Bioorg Med Chem 22, 3856-3861. Details PubMed

Chankhamjon P, Boettger-Schmidt D, Scherlach K, Urbansky B, Lackner G, Kalb D, Dahse HM, Hoffmeister D, Hertweck C (2014) Biosynthesis of the Halogenated Mycotoxin Aspirochlorine in Koji Mold Involves a Cryptic Amino Acid Conversion. Angew Chem Int Ed Engl 53, 13409-13413. Details PubMed

Hori C, Ishida T, Igarashi K, Samejima M, Suzuki H, Master E, Ferreira P, Ruiz-Dueñas FJ, Held B, Canessa P, Larrondo LF, Schmoll M, Druzhinina IS, Kubicek CP, Gaskell JA, Kersten P, St John F, Glasner J, Sabat G, Splinter BonDurant S, Syed K, Yadav J, Mgbeahuruike AC, Kovalchuk A, Asiegbu FO, Lackner G, Hoffmeister D, Rencoret J, Gutiérrez A, Sun H, Lindquist E, Barry K, Riley R, Grigoriev IV, Henrissat B, Kües U, Berka RM, Martínez AT, Covert SF, Blanchette RA, Cullen D (2014) Analysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood. PLoS Genet 10, e1004759. Details PubMed

Kalb D, Lackner G, Hoffmeister D (2014) Functional and phylogenetic divergence of fungal adenylate-forming reductases. Appl Environ Microbiol 80, 6175-6183. Details PubMed

Sasso S, Shelest E, Hoffmeister D (2014) Comments on the distribution and phylogeny of type I polyketide synthases and nonribosomal peptide synthetases in eukaryotes. Proc Natl Acad Sci U S A 111, E3946. Details PubMed

Schwenk D, Nett M, Dahse H-M, Horn U, Blanchette RA, Hoffmeister D (2014) Injury-induced biosynthesis of methyl-branched polyene pigments in a white-rotting basidiomycete J Nat Prod 77, 2658-2663. Details PubMed

Bohnert M, Dahse HM, Gibson DM, Krasnoff S, Hoffmeister D (2013) The fusarin analogue NG-391 impairs transcription in K-562 leukemia cells Phytochemistry Letters 6, 189-192. Details

Elliott CE, Callahan DL, Schwenk D, Nett M, Hoffmeister D, Howlett BJ (2013) A gene cluster responsible for biosynthesis of phomenoic acid in the plant pathogenic fungus, Leptosphaeria maculans. Fungal Genet Biol 53, 50-58. Details PubMed

Forseth RR, Amaike S, Schwenk D, Affeldt KJ, Hoffmeister D, Schroeder FC, Keller NP (2013) Homologous NRPS-like gene clusters mediate redundant small-molecule biosynthesis in Aspergillus flavus. Angew Chem Int Ed Engl 52, 1590-1594. Details PubMed

Kage H, Kreutzer MF, Wackler B, Hoffmeister D, Nett M (2013) An iterative type I polyketide synthase initiates the biosynthesis of the antimycoplasma agent micacocidin. Chem Biol 20, 764-771. Details PubMed

Kalb D, Lackner G, Hoffmeister D (2013) Fungal peptide synthetases – an update on functions and specificity signatures Fungal Biology Reviews 27, 43-50. Details

Lackner G, Bohnert M, Wick J, Hoffmeister D (2013) Assembly of melleolide antibiotics involves a polyketide synthase with cross-coupling activity. Chem Biol 20, 1101-1106. Details PubMed

Pauly J, Spiteller D, Linz J, Jacobs J, Allen C, Nett M, Hoffmeister D (2013) Ralfuranone thioether production by the plant pathogen Ralstonia solanacearum. Chembiochem 14, 2169-2178. Details PubMed

Steinchen W, Lackner G, Yasmin S, Schrettl M, Dahse HM, Haas H, Hoffmeister D (2013) Bimodular peptide synthetase SidE produces fumarylalanine in the human pathogen Aspergillus fumigatus. Appl Environ Microbiol 79, 6670-6676. Details PubMed

Fernandez-Fueyo E, Ruiz-Dueñas FJ, Ferreira P, Floudas D, Hibbett DS, Canessa P, Larrondo LF, James TY, Seelenfreund D, Lobos S, Polanco R, Tello M, Honda Y, Watanabe T, Watanabe T, Ryu JS, San RJ, Kubicek CP, Schmoll M, Gaskell J, Hammel KE, St John FJ, Vanden Wymelenberg A, Sabat G, Splinter BonDurant S, Syed K, Yadav JS, Doddapaneni H, Subramanian V, Lavín JL, Oguiza JA, Perez G, Pisabarro AG, Ramirez L, Santoyo F, Master E, Coutinho PM, Henrissat B, Lombard V, Magnuson JK, Kües U, Hori C, Igarashi K, Samejima M, Held BW, Barry KW, LaButti KM, Lapidus A, Lindquist EA, Lucas SM, Riley R, Salamov AA, Hoffmeister D, Schwenk D, Hadar Y, Yarden O, de Vries RP, Wiebenga A, Stenlid J, Eastwood D, Grigoriev IV, Berka RM, Blanchette RA, Kersten P, Martinez AT, Vicuna R, Cullen D (2012) Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis. Proc Natl Acad Sci U S A 109, 5458-5463. Details PubMed

Lackner G, Misiek M, Braesel J, Hoffmeister D (2012) Genome mining reveals the evolutionary origin and biosynthetic potential of basidiomycete polyketide synthases. Fungal Genet Biol 49, 996-991003. Details PubMed

Misiek M, Hoffmeister D (2012) Sesquiterpene aryl ester natural products in North American Armillaria species Mycol Progress 11, 7-15. Details

Wackler B, Lackner G, Chooi YH, Hoffmeister D (2012) Characterization of the Suillus grevillei quinone synthetase GreA supports a nonribosomal code for aromatic α-keto acids. Chembiochem 13, 1798-1804. Details PubMed

Baumgartner K, Coetzee MP, Hoffmeister D (2011) Secrets of the subterranean pathosystem of Armillaria. Mol Plant Pathol 12, 515-534. Details PubMed

Bohnert M, Miethbauer S, Dahse HM, Ziemen J, Nett M, Hoffmeister D (2011) In vitro cytotoxicity of melleolide antibiotics: structural and mechanistic aspects. Bioorg Med Chem Lett 21, 2003-2006. Details PubMed

Eastwood DC, Floudas D, Binder M, Majcherczyk A, Schneider P, Aerts A, Asiegbu FO, Baker SE, Barry K, Bendiksby M, Blumentritt M, Coutinho PM, Cullen D, de Vries RP, Gathman A, Goodell B, Henrissat B, Ihrmark K, Kauserud H, Kohler A, LaButti K, Lapidus A, Lavin JL, Lee YH, Lindquist E, Lilly W, Lucas S, Morin E, Murat C, Oguiza JA, Park J, Pisabarro AG, Riley R, Rosling A, Salamov A, Schmidt O, Schmutz J, Skrede I, Stenlid J, Wiebenga A, Xie X, Kües U, Hibbett DS, Hoffmeister D, Högberg N, Martin F, Grigoriev IV, Watkinson SC (2011) The plant cell wall-decomposing machinery underlies the functional diversity of forest fungi. Science 333, 762-765. Details PubMed

Kreutzer MF, Kage H, Gebhardt P, Wackler B, Saluz HP, Hoffmeister D, Nett M (2011) Biosynthesis of a complex yersiniabactin-like natural product via the mic locus in phytopathogen Ralstonia solanacearum. Appl Environ Microbiol 77, 6117-6124. Details PubMed

Misiek M, Braesel J, Hoffmeister D (2011) Characterisation of the ArmA adenylation domain implies a more diverse secondary metabolism in the genus Armillaria. Fungal Biol 115, 775-781. Details PubMed

Wackler B, Schneider P, Jacobs JM, Pauly J, Allen C, Nett M, Hoffmeister D (2011) Ralfuranone biosynthesis in Ralstonia solanacearum suggests functional divergence in the quinone synthetase family of enzymes. Chem Biol 18, 354-360. Details PubMed

Schneider P, Jacobs JM, Neres J, Aldrich CC, Allen C, Nett M, Hoffmeister D (2009) The global virulence regulators VsrAD and PhcA control secondary metabolism in the plant pathogen Ralstonia solanacearum. Chembiochem 10, 2730-2732. Details PubMed

Misiek M, Hoffmeister D (2008) Processing sites involved in intron splicing of Armillaria natural product genes. Mycol Res 112, 216-224. Details PubMed

Schneider P, Weber M, Hoffmeister D (2008) The Aspergillus nidulans enzyme TdiB catalyzes prenyltransfer to the precursor of bioactive asterriquinones. Fungal Genet Biol 45, 302-309. Details PubMed

Bouhired S, Weber M, Kempf-Sontag A, Keller NP, Hoffmeister D (2007) Accurate prediction of the Aspergillus nidulans terrequinone gene cluster boundaries using the transcriptional regulator LaeA. Fungal Genet Biol 44, 1134-1145. Details PubMed

Hoffmeister D, Keller NP (2007) Natural products of filamentous fungi: enzymes, genes, and their regulation. Nat Prod Rep 24, 393-416. Details PubMed

Misiek M, Hoffmeister D (2007) Fungal genetics, genomics, and secondary metabolites in pharmaceutical sciences. Planta Med 73, 103-115. Details PubMed

Schneider P, Weber M, Rosenberger K, Hoffmeister D (2007) A one-pot chemoenzymatic synthesis for the universal precursor of antidiabetes and antiviral bis-indolylquinones. Chem Biol 14, 635-644. Details PubMed

Bok JW, Hoffmeister D, Maggio-Hall LA, Murillo R, Glasner JD, Keller NP (2006) Genomic mining for Aspergillus natural products. Chem Biol 13, 31-37. Details PubMed

Chen YH, Wang CC, Greenwell L, Rix U, Hoffmeister D, Vining LC, Rohr J, Yang KQ (2005) Functional analyses of oxygenases in jadomycin biosynthesis and identification of JadH as a bifunctional oxygenase/dehydrase. J Biol Chem 280, 22508-22514. Details PubMed

Luzhetskyy A, Zhu L, Gibson M, Fedoryshyn M, Dürr C, Hofmann C, Hoffmeister D, Ostash B, Mattingly C, Adams V, Fedorenko V, Rohr J, Bechthold A (2005) Generation of novel landomycins M and O through targeted gene disruption. Chembiochem 6, 675-678. Details PubMed

Dürr C, Hoffmeister D, Wohlert SE, Ichinose K, Weber M, Von Mulert U, Thorson JS, Bechthold A (2004) The glycosyltransferase UrdGT2 catalyzes both C- and O-glycosidic sugar transfers. Angew Chem Int Ed Engl 43, 2962-2965. Details PubMed

Hoffmeister D, Thorson JS (2004) Mechanistic implications of Escherichia coli galactokinase structure-based engineering. Chembiochem 5, 989-992. Details PubMed

Hoffmeister D, Weber M, Dräger G, Ichinose K, Dürr C, Bechthold A (2004) Rational saccharide extension by using the natural product glycosyltransferase LanGT4. Chembiochem 5, 369-371. Details PubMed

Thorson JS, Barton WA, Hoffmeister D, Albermann C, Nikolov DB (2004) Structure-based enzyme engineering and its impact on in vitro glycorandomization. Chembiochem 5, 16-25. Details PubMed

Hoffmeister D, Dräger G, Ichinose K, Rohr J, Bechthold A (2003) The C-Glycosyltransferase UrdGT2 is unselective toward d- and l-configured nucleotide-bound rhodinoses. J Am Chem Soc 125, 4678-4679. Details PubMed

Hoffmeister D, Yang J, Liu L, Thorson JS (2003) Creation of the first anomeric D/L-sugar kinase by means of directed evolution. Proc Natl Acad Sci U S A 100, 13184-13189. Details PubMed

Pelzer S, Hoffmeister D, Merfort I, Bechthold A (2003) Procaryotic and eucaryotic cells in biotech production In: Kayser O, Müller R. H. (eds.) Pharmaceutical Biotechnology, Drug Discovery and Clinical Applications pp. 9-33. Wiley-Blackwell. Details

Rebets Y, Ostash B, Luzhetskyy A, Hoffmeister D, Brana A, Mendez C, Salas JA, Bechthold A, Fedorenko V (2003) Production of landomycins in Streptomyces globisporus 1912 and S cyanogenus S136 is regulated by genes encoding putative transcriptional activators. FEMS Microbiol Lett 222, 149-153. Details PubMed

Rix U, Remsing LL, Hoffmeister D, Bechthold A, Rohr J (2003) Urdamycin L: a novel metabolic shunt product that provides evidence for the role of the urdM gene in the urdamycin A biosynthetic pathway of Streptomyces fradiae TU 2717. Chembiochem 4, 109-111. Details PubMed

Hoffmeister D, Wilkinson B, Foster G, Sidebottom PJ, Ichinose K, Bechthold A (2002) Engineered urdamycin glycosyltransferases are broadened and altered in substrate specificity. Chem Biol 9, 287-295. Details PubMed

Hoffmeister D, Ichinose K, Bechthold A (2001) Two sequence elements of glycosyltransferases involved in urdamycin biosynthesis are responsible for substrate specificity and enzymatic activity. Chem Biol 8, 557-567. Details PubMed

Weitnauer G, Mühlenweg A, Trefzer A, Hoffmeister D, Süssmuth RD, Jung G, Welzel K, Vente A, Girreser U, Bechthold A (2001) Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tü57 and production of new antibiotics. Chem Biol 8, 569-581. Details PubMed

Faust B, Hoffmeister D, Weitnauer G, Westrich L, Haag S, Schneider P, Decker H, Künzel E, Rohr J, Bechthold A (2000) Two new tailoring enzymes, a glycosyltransferase and an oxygenase, involved in biosynthesis of the angucycline antibiotic urdamycin A in Streptomyces fradiae Tü2717. Microbiology 146 ( Pt 1), 147-154. Details PubMed

Hoffmeister D, Ichinose K, Domann S, Faust B, Trefzer A, Dräger G, Kirschning A, Fischer C, Künzel E, Bearden D, Rohr J, Bechthold A (2000) The NDP-sugar co-substrate concentration and the enzyme expression level influence the substrate specificity of glycosyltransferases: cloning and characterization of deoxysugar biosynthetic genes of the urdamycin biosynthetic gene cluster. Chem Biol 7, 821-831. Details PubMed

Trefzer A, Hoffmeister D, Künzel E, Stockert S, Weitnauer G, Westrich L, Rix U, Fuchser J, Bindseil KU, Rohr J, Bechthold A (2000) Function of glycosyltransferase genes involved in urdamycin A biosynthesis. Chem Biol 7, 133-142. Details PubMed