Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9.

Jiao C, Sharma S, Dugar G, Peeck NL, Bischler T, Wimmer F, Yu Y, Barquist L, Schoen C, Kurzai O, Sharma CM, Beisel CL (2021) Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9. Science 372(6545), 941-948.

Abstract

CRISPR-Cas systems recognize foreign genetic material using CRISPR RNAs (crRNAs). In type II systems, a trans-activating crRNA (tracrRNA) hybridizes to crRNAs to drive their processing and utilization by Cas9. While analyzing Cas9-RNA complexes from Campylobacter jejuni, we discovered tracrRNA hybridizing to cellular RNAs, leading to formation of

Leibniz-HKI-Autor*innen

Oliver Kurzai

Identifier

doi: 10.1126/science.abe7106

PMID: 33906967