Lars Regestein
Forschungsschwerpunkte
  • Entwicklung von Online-Messverfahren (bspw. Reaktorkalorimetrie) zur detaillierten Charakterisierung biotechnologischer Prozesse          
  • Kultivierung von (hoch-)viskosen biologischen Systemen zur Herstellung von Biopolymeren       
  • Etablierung und Regelung von mikrobiellen Mischkulturen
Curriculum vitae

Wissenschaftlicher Werdegang

Seit 12/2017 Stellvertretender Abteilungsleiter Biotechnikum, Leibniz Institut für Naturstoff-Forschung und Infektionsbiologie, (HKI) Jena
2015 - 2017 Oberingenieur, Aachener Verfahrenstechnik – Lehrstuhl für Bioverfahrenstechnik, RWTH Aachen
2014 – 2015 Forschungsaufenthalt als Research Professor Western University, London, Ontario, Kanada
2012 - 2014 Leitender wissenschaftlicher Angestellter (Oberingenieur) Aachener Verfahrenstechnik, RWTH Aachen
2007 – 2011 Promotion, RWTH Aachen University
2000 – 2007 Diplomstudium der Verarbeitungs- und Verfahrenstechnik, TU Dresden

Auszeichnungen · Ämter · wissenschaftliche Aktivitäten

2014 - 2015 Theodore von Kármán Fellowship (RWTH Aachen University)
2014 - 2015 Western Fellowship(Western University, London, Ontario)
Seit 2017 Zukunftsforum Biotechnologie (DECHEMA)
Seit 2014 BioFuelNet Canada
Seit 2007 Netzwerk Bioverfahrenstechnik Dresden e. V.
Publikationen

Fricke J, Kargbo R, Regestein L, Lenz C, Peschel G, Rosenbaum MA, Sherwood A, Hoffmeister D (2020) Scalable hybrid synthetic/biocatalytic route to psilocybin. Chem Eur J 37(26), 8281-8285. Details PubMed Open Access PDF

Guo H, Schwitalla JW, Benndorf R, Baunach M, Steinbeck C, Görls H, de Beer ZW, Regestein L, Beemelmanns C (2020) Gene cluster activation in a bacterial symbiont leads to halogenated angucyclic maduralactomycins and spirocyclic actinospirols. Org Lett 22(7), 2634-2638. Details PubMed

Kufs JE, Hoefgen S, Rautschek J, Bissell AU, Graf C, Fiedler J, Braga D, Regestein L, Rosenbaum MA, Thiele J, Valiante V (2020) Rational design of flavonoid production routes using combinatorial and precursor-directed biosynthesis. ACS Synth Biol 9(7), 1823-1832. Details PubMed Open Access PDF

Munch G, Schulte A, Mann M, Dinger R, Regestein L, Rehmann L, Büchs J (2020) Online measurement of CO2and total gas production in parallel anaerobic shake flask cultivations. Biochem Eng J 153, 107418. Details Open Access PDF

Peña-Ortiz L, Graça AP, Guo H, Braga D, Köllner TG, Regestein L, Beemelmanns C, Lackner G (2020) Structure elucidation of the redox cofactor mycofactocin reveals oligo-glycosylation by MftF. Chem Sci 11, 5182-5190. Details Open Access PDF

Geinitz B, Rehman L, Büchs J, Regestein L (2019) Noninvasive tool for optical online monitoring of individual biomass concentrations in a defined coculture. Biotechnol Bioeng 117(4), 999-1011. Details PubMed Open Access PDF

Heyman B, Lamm R, Tulke H, Regestein L, Büchs J (2019) Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production. Microb Cell Fact 18(1), 78. Details PubMed Open Access PDF

Peña-Ortiz L, Graça A P, Guo H, Braga D, Köllner T G, Regestein L, Beemelmanns C, Lackner G (2019) Biosynthesis of the redox cofactor mycofactocin comprises oligoglycosylation by MftF in Mycolicibacterium smegmatis. bioRxiv , Details Open Access PDF

Trottmann F, Franke J, Richter I, Ishida K, Cyrulies M, Dahse HM, Regestein L, Hertweck C (2019) Cyclopropanol warhead in malleicyprol confers virulence of human- and animal-pathogenic Burkholderia species. Angew Chem Int Ed 58(40), 14129-14133. Details PubMed Open Access PDF

Kreyenschulte D, Heyman B, Eggert A, Maßmann T, Kalvelage C, Kossack R, Regestein L, Jupke A, Büchs J. (2018) In situ reactive extraction of itaconic acid during fermentation of Aspergillus terreus. Biochem Eng J 135, 133-141. Details

Regestein L, Klement T, Grande P, Kreyenschulte D, Heyman B, Maßmann T, Eggert A, Sengpiel R, Wang Y, Wierckx N, Blank LM, Spiess A, Leitner W, Bolm C, Wessling M, Jupke A, Rosenbaum M, Büchs J (2018) From beech wood to itaconic acid: Case study on biorefinery process integration. Biotechnol Biofuels 11, 279. Details PubMed Open Access PDF

Antonov E, Schlembach I, Regestein L, Rosenbaum MA, Büchs J (2017) Process relevant screening of cellulolytic organisms for consolidated bioprocessing. Biotechnol Biofuels 10, 106. Details PubMed

Lara AR, Jaén KE, Sigala JC, Mühlmann M, Regestein L, Büchs J (2017) Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli. ACS Synth Biol 6(2), 344-356. Details PubMed

Lara AR, Jaén KE, Sigala JC, Regestein L, Büchs J (2017) Evaluation of microbial globin promoters for oxygen-limited processes using Escherichia coli. J Biol Eng 11, 39. Details PubMed

Schelden M, Lima W, Doerr E, Wunderlich M, Rehmann L, Büchs J, Regestein L (2017) Online measurement of viscosity for xanthan production in stirred tank reactors. Biotechnology and Bioengineering 114(5), 990-997. Details

Schlembach I, Regestein L, Rosenbaum MA (2017) Etablierung filamentöser Mischkulturen für Bioprozesse. BIOSpectrum 23(3), 270-272. Details Open Access

Antonov E, Wirth S, Gerlach T, Schlembach I, Rosenbaum MA, Regestein L, Büchs J (2016) Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks. Microb Cell Fact 15(1), 164. Details PubMed

Kreyenschulte D, Emde F, Regestein L, Büchs J (2016) Computational minimization of the specific energy demand of large-scale aerobic fermentation processes based on small-scale data. Chemical Engineering Science 153, 270-283. Details

Meier K, Carstensen F, Wessling M, Regestein L, Büchs L (2016) Quasi-continuous fermentation in a reverse-flow diafiltration bioreactor. Biochemical Engineering Journal 91, 265-275. Details

Meier K, Klöckner W, Bonhage B, Antonov E, Regestein L, Büchs J (2016) Correlation for the maximum oxygen transfer capacity in shake flasks for a wide range of operating conditions and for different culture media. Biochemical Engineering Journal 109, 228-235. Details

Sieben M, Steinhorn G, Müller C, Fuchs S, Ann Chin L, Regestein L, Büchs J (2016) Testing plasmid stability of Escherichia coli using the Continuously Operated Shaken BIOreactor System. Biotechnol Prog 32(6), 1418-1425. Details PubMed

Wunderlich M, Trampnau PP, Lopes EF, Büchs L, Regestein L (2016) Online in situ viscosity determination in stirred tank reactors by measurement of the heat transfer capacity Chemical Engineering Science 152, 116-126. Details

Kreyenschulte D, Paciok E, Regestein L, Blümich B, Büchs J (2015) Online monitoring of fermentation processes via non-invasive low-field NMR. Biotechnol Bioeng 112(9), 1810-1821. Details PubMed

Regestein L, Doerr EW, Staaden A, Rehmann L (2015) Impact of butyric acid on butanol formation by Clostridium pasteurianum. Bioresour Technol 196, 153-159. Details PubMed

Wewetzer SJ, Kunze M, Ladner T, Luchterhand B, Roth S, Rahmen N, Kloß R, Costa E Silva A, Regestein L, Büchs J (2015) Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors. J Biol Eng 9, 9. Details PubMed

Meier K, Carstensen F, Scheeren C, Regestein L, Wessling M, Büchs J (2014) In situ product recovery of single-chain antibodies in a membrane bioreactor. Biotechnol Bioeng 111(8), 1566-1576. Details PubMed

Meier K, Carstensen F, Wessling M, Regestein L, Büchs j (2014) Quasi-continuous fermentation in a reverse-flow diafiltration bioreactor Biochemical Engineering Journal 91, 265-275. Details

Meier K, Djeljadini S, Regestein L, Büchs J, Carstensen F, Wessling M, Holland T, Raven N (2014) In situ cell retention of a CHO culture by a reverse-flow diafiltration membrane bioreactor. Biotechnol Prog 30(6), 1348-1355. Details PubMed

Meier K, Herweg E, Schmidt B, Klement T, Regestein L, Büchs L (2013) Quantifying the release of polymer additives from single-use materials by respiration activity monitoring Polymer Tesing 32(6), 1064-1071. Details

Regestein L, Giese H, Zavrel M, Büchs J (2013) Comparison of two methods for designing calorimeters using stirred tank reactors. Biotechnol Bioeng 110(1), 180-190. Details PubMed

Regestein L, Maskow T, Tack A, Knabben I, Wunderlich M, Lerchner J, Büchs J (2013) Non-invasive online detection of microbial lysine formation in stirred tank bioreactors by using calorespirometry. Biotechnol Bioeng 110(5), 1386-1395. Details PubMed

Regestein L, Wolf A, Schneider HJ, Maskow T, Mertens F, Büchs J (2012) A chip calorimeter for the monitoring of conventional bioreactors at elevated cell concentrations Thermochimica Acta 544, 10-16. Details

Lara AR, Knabben I, Regestein L, Sassi J, Caspeta L, Ramírez OT (2011) Comparison of oxygen enriched air vs. pressure cultivations to increase oxygen transfer and to scale-up plasmid DNA production fermentations Engineering in Life Sciences 11(4), 382-386. Details

Maskow T, Schubert T, Wolf A, Buchholz F, Regestein L, Buechs J, Mertens F, Harms H, Lerchner J (2011) Potentials and limitations of miniaturized calorimeters for bioprocess monitoring. Appl Microbiol Biotechnol 92(1), 55-66. Details PubMed

Schmidt JK, Riedele C, Regestein L, Rausenberger J, Reichl U (2011) A novel concept combining experimental and mathematical analysis for the identification of unknown interspecies effects in a mixed culture. Biotechnol Bioeng 108(8), 1900-1911. Details PubMed

Knabben I, Regestein L, Grumbach C, Steinbusch S, Kunze G, Büchs J (2010) Online determination of viable biomass up to very high cell densities in Arxula adeninivorans fermentations using an impedance signal. J Biotechnol 149(1-2), 60-66. Details PubMed

Knabben I, Regestein L, Marquering F, Steinbusch S, Lara AR, Büchs J (2010) High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor. J Biotechnol 150(1), 73-79. Details PubMed