Release-activated Ca2+ transport in neurons of frog sympathetic ganglia.

Cseresnyés Z, Bustamante AI, Klein MG, Schneider MF (1997) Release-activated Ca2+ transport in neurons of frog sympathetic ganglia. Neuron 19(2), 403-419.

Abstract

Frog sympathetic ganglion neurons exhibit a novel Ca2+ uptake mechanism, release-activated calcium transport or RACT, which is manifest in both cytosolic and store [Ca2+] signals as greatly accelerated Ca2+ uptake after Ca2+ release from internal stores. RACT is activated by Ca2+ release but not by Ca2+ entry and serves to selectively refill Ca2+ stores after release. RACT lowers cytosolic [Ca2+] with a rate constant about 1.6 times that of the SERCA pump with empty ER. RACT is thapsigargin-insensitive, was eliminated by ryanodine, but was not affected by blocking mitochondrial or plasma membrane Ca2+ transport. A Ca2+ flux model with RACT in the ER membrane reproduced the cytosolic and store [Ca2+] responses to all stimuli.

Leibniz-HKI-Autor*innen

Zoltán Cseresnyés

Identifier

PMID: 9292729