Implementation of 89Zr production and in vivo imaging of B-cells in mice with 89Zr-labeled anti-B-cell antibodies by small animal PET/CT.

Walther M, Gebhardt P, Grosse-Gehling P, Würbach L, Irmler I, Preusche S, Khalid M, Opfermann T, Kamradt T, Steinbach J, Saluz HP (2011) Implementation of 89Zr production and in vivo imaging of B-cells in mice with 89Zr-labeled anti-B-cell antibodies by small animal PET/CT. Appl Radiat Isot 69(6), 852-857.

Abstract

We examined the production, separation, and characterization of (89)Zr, including supplementation of a commercial Cyclone(®) 18/9 with a self-made Solid Target System (STS). Obtained [(89)Zr]Zr-oxalate was used for the labeling of anti-B cell antibodies with desferrioxamine-p-SCN as a bifunctional chelator. (89)Zr-labeled antibodies were injected in DBA/1 mice to examine usability for detection of B cells in vivo. PET measurements showed binding of (89)Zr-labeled anti-B cell antibodies in tissues with high frequencies of B cells, i.e. in spleen and lymph nodes.

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Leibniz-HKI-Autor*innen

Hans Peter Saluz

Identifier

doi: 10.1016/j.apradiso.2011.02.040

PMID: 21397511