Dr. Daniel Last



Hasan M, Schulze S, Berndt L, Palm G J, Braga D, Richter I, Last D, Lammers M, Lackner G# (2022) Diversification by CofC and control by CofD govern biosynthesis and evolution of coenzyme F420 and its derivative 3PG-F420. mBio 13(1), e03501-21.
Last D, Hasan M, Rothenburger L, Braga D, Lackner G# (2022) High-yield production of coenzyme F420 in Escherichia coli by fluorescence-based screening of multi-dimensional gene expression space. Metab Eng 73, 158-167.
Braga D, Hasan M, Kröber T, Last D, Lackner G (2020) Biosynthesis of the redox coenzyme F420 in Thermomicrobia involves reduction by standalone nitroreductase superfamily enzymes. Appl Environ Microbiol 86(12), e00457-20.
Braga D, Last D, Hasan M, Guo H, Leichnitz D, Uzum Z, Richter I, Schalk F, Beemelmanns C, Hertweck C, Lackner G (2019) Metabolic pathway rerouting in Paraburkholderia rhizoxinica evolved long-overlooked derivatives of coenzyme F420. ACS Chem Biol 14(9), 2088-2094.
Dörr M, Fibinger MP, Last D, Schmidt S, Santos-Aberturas J, Böttcher D, Hummel A, Vickers C, Voss M, Bornscheuer UT (2016) Fully automatized high-throughput enzyme library screening using a robotic platform. Biotechnol Bioeng 113(7), 1421-1432.
Last D, Krüger GH, Dörr M, Bornscheuer UT (2016) Fast, Continuous, and High-Throughput (Bio)Chemical Activity Assay for N-Acyl-l-Homoserine Lactone Quorum-Quenching Enzymes. Appl Environ Microbiol 82(14), 4145-4154.
Last D, Müller J, Dawood AWH, Moldenhauer EJ, Pavlidis IV, Bornscheuer UT (2016) Highly efficient and easy protease-mediated protein purification. Appl Microbiol Biotechnol 100(4), 1945-1953.