The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus.

da Silva Ferreira ME, Kress MR, Savoldi M, Goldman MH, Härtl A, Heinekamp T, Brakhage AA, Goldman GH (2006) The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryot Cell 5(1), 207-211. PubMed

Abstract

To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB(KU80)). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuB(KU80) mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB(KU80) had no influence on pathogenicity in a low-dose murine infection model.

Beteiligte Abteilungen und Gruppen

Mikrobielle Pathogenitätsmechanismen Molekulare und Angewandte Mikrobiologie

HKI-Autoren

Prof. Dr. Axel A. Brakhage Dr. Thorsten Heinekamp

Identifier

PMID: 16400184