Background: Nitroxoline is an old antibiotic currently approved for the treatment of uncomplicated urinary tract infections. Its mode of action is based on the chelation of bivalent cations, such as zinc.

Objectives: As zinc plays a central role in the metabolism of Aspergillus spp., we performed antifungal susceptibility testing against Nitroxoline using broth microdilution.

Methods: Overall, 13 Aspergillus isolates were tested according to the EUCAST guidance document (Guinea et al. EUCAST, def. doc. 9.4, 2022) including two genetically proven Azole-resistant strains.

Results: The overall Minimal Inhibitory Concentration (MIC)_50/90 was 0.5 mg/L.

Conclusions: These results correspond to excellent in vitro activity against Aspergillus spp.