Purification of infectious and non-infectious chlamydial particles using iodixanol for density gradient preparation.

Beder T, Scheven MT, Praetzsch D, Westermann M, Saluz HP (2016) Purification of infectious and non-infectious chlamydial particles using iodixanol for density gradient preparation. J Microbiol Methods 128, 20-23.

Abstract

Chlamydiae are obligate intracellular bacteria with two distinct morphological stages, the infectious elementary bodies (EBs) and non-infectious reticulate bodies (RBs). Here we describe a rapid and straightforward protocol for the purification of EBs and RBs involving special density gradients. It has been successfully applied to three chlamydial species.

Leibniz-HKI-Autoren

Hans Peter Saluz
Thomas Beder
Mareike Scheven

Identifier

doi: 10.1016/j.mimet.2016.06.029

PMID: 27378442