BEGIN:VCALENDAR PRODID:-//eluceo/ical//2.0/EN VERSION:2.0 CALSCALE:GREGORIAN BEGIN:VEVENT UID:d1437631b1089eec416becdaeaf70479 DTSTAMP:20240302T110718Z SUMMARY:A proteomics approach to understand physiology and virulence of Sta phylococcus aureus DESCRIPTION:Staphylococcus aureus is a human pathogen of strong clinical si gnificance due to increasing infections with multi-resistant isolates. A b etter understanding of its infection biology is urgently required to comba t this pathogen also in the future. The fitness of this pathogen\, which i s based on its ability to successfully adapt to host conditions\, is cruci al for full virulence. During colonization and infection\, S. aureus is co nfronted with a multitude of signals including growth-limiting factors and life-threatening host defense mechanisms. Therefore\, adaptation of bacte rial gene expression in their natural habitat is a multi-signal response a nd it will be a challenging task for future studies to unravel this divers e network. We established an S. aureus protein expression database Aureoli b containing 4\,692 time dependent expression profiles for 521 cytoplasmic proteins under nine infection relevant\, well defined laboratory conditio ns. Initially\, this data base was used as a tool box to get new insights into S. aureus adaptation to more complex\, infection-relevant experimenta l models such as nasal secretions\, milk and macrophages. For that purpose we used a green fluorescence protein (GFP) expressing S. aureus Newman st rain and analyzed its expression of intracellular and surface associated p roteins under these conditions by mass spectrometry in combination with is otopic protein labeling. We found evidence that under these conditions S. aureus copes with various nutrient limitations (e. g. carbon and iron sour ces).  Moreover\, we found that proteins necessary for tissue adhesion an d immune evasion are preferentially expressed in cells grown in nasal secr etions and macrophages whereas toxins and exoenzymes are repressed at the same time. URL: DTSTART:20151104T190000 DTEND:20151104T190000 LOCATION:Großer Hörsaal Erbertstraße END:VEVENT BEGIN:VTIMEZONE TZID:Europe/Berlin BEGIN:STANDARD DTSTART:20151104T190000 TZNAME:CET TZOFFSETTO:+0100 TZOFFSETFROM:+0100 END:STANDARD END:VTIMEZONE END:VCALENDAR