AoiQ catalyzes geminal dichlorination of 1,3-diketone natural products.

Liu M, Ohashi M, Hung YS, Scherlach K, Watanabe K, Hertweck C, Tang Y (2021) AoiQ catalyzes geminal dichlorination of 1,3-diketone natural products. J Am Chem Soc 143(19), 7267-7271.

Abstract

Enzymes that can perform halogenation of aliphatic carbons are of significant interest to the synthetic and biocatalysis communities. Here we describe the characterization of AoiQ, a single-component flavin-dependent halogenase (FDH) that catalyzes gem-dichlorination of 1,3-diketone substrates in the biosynthesis of dichlorodiaporthin. AoiQ represents the first biochemically reconstituted FDH that can halogenate an enolizable sp3-hybridized carbon atom.

Leibniz-HKI-Authors

Christian Hertweck
Kirstin Scherlach

Identifier

doi: 10.1021/jacs.1c02868

PMID: 33957045