A type III polyketide synthase specific for sporulating negativicutes is responsible for alkylpyrone biosynthesis.

Bredy F, Ishida K, Hertweck C (2022) A type III polyketide synthase specific for sporulating negativicutes is responsible for alkylpyrone biosynthesis. Chembiochem , e202200431.

Abstract

Genomic analyses indicate that anaerobic bacteria represent a neglected source of natural products. Whereas a limited number of polyketides have been reported from anaerobes, products of type III polyketide synthases (PKSs) have remained unknown. We found a highly conserved biosynthetic gene cluster (BGC) comprising genes putatively encoding a type III PKS and a methyltransferase in genomes of the Negativicutes, strictly anaerobic, diderm bacteria. By in vivo and in vitro expression of a type III PKS gene, dquA from the oak-associated Dendrosporobacter quercicolus in E. coli we show production of long-chain alkylpyrones. Intriguingly, this BGC is specific for sporulating Sporomusaceae but absent in related Negativicutes that do not sporulate, thus suggesting a physiological role.

Leibniz-HKI-Authors

Florian Bredy
Christian Hertweck
Keishi Ishida

Identifier

doi: 10.1002/cbic.202200431

PMID: 35997218