Novel biocatalysts from specialized metabolism.

Kries H, Trottmann F, Hertweck C (2023) Novel biocatalysts from specialized metabolism. Angew Chem Int Ed Engl 63(4), e202309284. (Review)


Enzymes are increasingly recognized as valuable (bio)catalysts that complement existing synthetic methods. However, the range of biotransformations used in the laboratory is limited. Here we give an overview on the biosynthesis-inspired discovery of novel biocatalysts that address various synthetic challenges. Prominent examples from this dynamic field highlight remarkable enzymes for protecting-group-free amide formation and modification, control of pericyclic reactions, achieve stereoselective hetero- and polycyclizations, enable atroposelective aryl couplings, facilitate site-selective C-H activations, introduce ring strain, and promote N-N bond formations. We also explore unusual functions of cytochrome P450 monooxygenases, radical SAM-dependent enzymes, flavoproteins, and enzymes recruited from primary metabolism, which offer opportunities for synthetic biology, enzyme engineering, directed evolution, and catalyst design.


Christian Hertweck
Hajo Kries
Felix Trottmann


doi: 10.1002/anie.202309284

PMID: 37737720