Immunomodulatory function of antimicrobial peptide Ec-Hepcidin1 modulates the induction of inflammatory gene expression in primary cells of caspian trout (Salmo trutta caspius Kessler, 1877).

Ghodsi Z, Kalbassi MR, Farzaneh P, Mobarez AM, Beemelmanns C, Amiri Moghaddam J (2020) Immunomodulatory function of antimicrobial peptide Ec-Hepcidin1 modulates the induction of inflammatory gene expression in primary cells of caspian trout (Salmo trutta caspius Kessler, 1877). Fish Shellfish Immunol 104, 55-61.

Abstract

Hepcidins, a group of antimicrobial peptides (AMPs), play a key role in the innate immune system of fishes and act against different pathogens. In this study, antimicrobial and immune-inflammatory activity of a synthetic Ec-hepcidin1, previously identified from orange-spotted grouper, were evaluated. EC-hepcidin1 showed weak activity against the zoonotic fish pathogen Streptococcus iniae (MIC 100 μg mL−1 and MBC 150 μg mL−1). To study the effect of AMPs in general, and Ec-hepcidin1 in particular, a primary cell culture (SC) from the fin tissue of the Caspian Trout (Salmo trutta caspius) was established. The neutral Red method on SC cells revealed that Ec-hepcidin1 has no or very low cytotoxic properties. Treatment of cells with either EC-hepcidin1(150 μg mL−1) or fish pathogen Streptococcus iniae (MOI = 10) and a mixture of both resulted in the up-regulation of gene expression of MHC-UBA, IL-6, and TNFα indicating the modulatory function on inflammatory processes. These findings indicate that EC-hepcidin1 might act as a candidate for modulation of the innate immune system in S. iniae-based infection.

Leibniz-HKI-Authors

Christine Beemelmanns
Jamshid Amiri Moghaddam

Identifier

doi: 10.1016/j.fsi.2020.05.067

PMID: 32473358