Cryptic gene clusters

Learning from Picornaviruses, we exploited 2A peptides to express entire biosynthetic pathways as single polycistronic genes. Assembling plasmids and read out systems were developed in order to efficiently screen transformed recipient organisms.

Cryptic gene clusters


Fabio Gherlone


Bissell AU, Rautschek J, Hoefgen S, Raguž L, Mattern DJ, Saeed N, Janevska S, Jojić K, Huang Y, Kufs JE, Herboeck B, Guo H, Hillmann F, Beemelmanns C, Valiante V (2022) Biosynthesis of the sphingolipid inhibitors sphingofungins in filamentous fungi requires aminomalonate as a metabolic precursor. ACS Chem Biol 17(2), 386-394.
Huang Y*, Hoefgen S*, Gherlone F, Valiante V (2022) Intrinsic ability of the ß-oxidation pathway to produce bioactive styrylpyrones. Angew Chem Int Ed 61(34), e202206851.
Mattern DJ, Valiante V, Horn F, Petzke L, Brakhage AA (2017) Rewiring of the Austinoid Biosynthetic Pathway in Filamentous Fungi. ACS Chem Biol 12(12), 2927-2933.