(2022)
Biosynthesis of the sphingolipid inhibitors sphingofungins in filamentous fungi requires aminomalonate as a metabolic precursor.
ACS Chem Biol 17(2),
386-394.
Cryptic gene clusters
Learning from Picornaviruses, we exploited 2A peptides to express entire biosynthetic pathways as single polycistronic genes. Assembling plasmids and read out systems were developed in order to efficiently screen transformed recipient organisms.
