Ralfuranone is produced by an alternative aryl-substituted γ-lactone biosynthetic route in Ralstonia solanacearum.
(2014) Ralfuranone is produced by an alternative aryl-substituted γ-lactone biosynthetic route in Ralstonia solanacearum. J Nat Prod 77, 1967-1971.
The aryl-substituted γ-lactones ralfuranones A and B were isolated after feeding l-[1-(13)C]-phenylalanine to a liquid culture of the plant pathogenic bacterium Ralstonia solanacearum. (13)C NMR analysis demonstrated specific enrichment of the label at position 2 of the γ-lactone. This labeling pattern is consistent with a biosynthetic mechanism that includes direct cyclization of two monomeric phenylpyruvate precursors into an α,β-substituted lactone, but incompatible with a terphenylquinone intermediate. As the latter was shown as an intermediate in allantofuranone biosynthesis, we conclude that aryl-substituted γ-lactones can be assembled via divergent biosynthetic routes.