Oak-associated negativicute equipped with ancestral aromatic polyketide synthase produces antimycobacterial dendrubins.

Ishida K, Shabuer G, Schieferdecker S, Pidot SJ, Stinear TP, Knuepfer U, Cyrulies M, Hertweck C (2020) Oak-associated negativicute equipped with ancestral aromatic polyketide synthase produces antimycobacterial dendrubins. Chem Eur J 26(58), 13147-13151.

Abstract

Anaerobic bacteria have only recently been recognized as a source of antibiotics; yet, the metabolic potential of Negativicutes (Gram-negative staining Firmicutes) such as the oak-associated Dendrosporobacter quercicolus has remained unknown. Genome mining of D. quercicolus and phylogenetic analyses revealed a gene cluster for a type II polyketide synthase (PKS) complex that belongs to the most ancestral enzyme systems of this type. Metabolic profiling, NMR analyses, and stable-isotope labeling led to the discovery of a new family of anthraquinone-type polyphenols, the dendrubins, which are diversified by acylation, methylation and dimerization. Dendrubin A and B were identified as strong antibiotics against a range of clinically relevant, human-pathogenic mycobacteria.

Leibniz-HKI-Authors

Michael Cyrulies
Christian Hertweck
Keishi Ishida
Uwe Knüpfer
Sebastian Schieferdecker
Gulimila Shabuer

Identifier

doi: 10.1002/chem.202001939

PMID: 32597507