A laminar-flow microfluidic device for quantitative analysis of microbial electrochemical activity.

Li Z, Venkataraman A, Rosenbaum MA, Angenent LT (2012) A laminar-flow microfluidic device for quantitative analysis of microbial electrochemical activity. ChemSusChem 5(6), 1119-1123. PubMed

Abstract

We report a laminar flow-based microfluidic bioelectrochemical system (BES) that was fabricated by using polydimethyl siloxane (PDMS) channels and gold electrodes. The microfluidic BES was operated as a potentiostatically controlled two-electrode system. A pure culture of Geobacter sulfurreducens strain PCA, which is a model electrode-respiring bacterium, was grown in the channel and respired with the electrode under strict anaerobic conditions. We took advantage of the short hydraulic retention time (≈ 2 min) and response times (

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doi: 10.1002/cssc.201100736 PMID: 22674693