Structure elucidation of the redox cofactor mycofactocin reveals oligo-glycosylation by MftF.

Peña-Ortiz L, Graça AP, Guo H, Braga D, Köllner TG, Regestein L, Beemelmanns C, Lackner G (2020) Structure elucidation of the redox cofactor mycofactocin reveals oligo-glycosylation by MftF. Chem Sci 11, 5182-5190. Open Access PDF

This article is part of the themed collection "2020 Chemical Science HOT Article Collection" and was highlighted on the back cover of Chemical Science.

Abstract

Mycofactocin (MFT) is a redox cofactor belonging to the family of ribosomally synthesized and post-translationally modified peptides (RiPPs) and is involved in alcohol metabolism of mycobacteria including Mycobacterium tuberculosis. A preliminary biosynthetic model had been established by bioinformatics and in-vitro studies, while the structure of natural MFT and key biosynthetic steps remained elusive. Here, we report the discovery of glycosylated MFT by 13C-labeling metabolomics and establish a model of its biosynthesis in Mycolicibacterium smegmatis. Extensive structure elucidation including NMR revealed that MFT is decorated with up to nine β-1,4-linked glucose residues including 2-O-methylglucose. Dissection of biosynthetic genes demonstrated that the oligoglycosylation is catalyzed by the glycosyltransferase MftF. Furthermore, we confirm the redox cofactor function of glycosylated MFTs by activity-based metabolic profiling using the carveol dehydrogenase LimC and show that the MFT pool expands during cultivation on ethanol. Our results will guide future studies into the biochemical functions and physiological roles of MFT in bacteria.

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doi: 10.1039/D0SC01172J