Oak-associated negativicute equipped with ancestral aromatic polyketide synthase produces antimycobacterial dendrubins.

Ishida K, Shabuer G, Schieferdecker S, Pidot SJ, Stinear TP, Knuepfer U, Cyrulies M, Hertweck C (2020) Oak-associated negativicute equipped with ancestral aromatic polyketide synthase produces antimycobacterial dendrubins. Chem Eur J [Epub ahead of print] PubMed Open Access PDF

Abstract

Anaerobic bacteria have only recently been recognized as a source of antibiotics; yet, the metabolic potential of Negativicutes (Gram-negative staining Firmicutes) such as the oak-associated Dendrosporobacter quercicolus has remained unknown. Genome mining of D. quercicolus and phylogenetic analyses revealed a gene cluster for a type II polyketide synthase (PKS) complex that belongs to the most ancestral enzyme systems of this type. Metabolic profiling, NMR analyses, and stable-isotope labeling led to the discovery of a new family of anthraquinone-type polyphenols, the dendrubins, which are diversified by acylation, methylation and dimerization. Dendrubin A and B were identified as strong antibiotics against a range of clinically relevant, human-pathogenic mycobacteria.

Involved Units and Groups
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doi: 10.1002/chem.202001939 PMID: 32597507