Daniel H. Scharf

Dr. Daniel H. Scharf

Molecular and Applied Microbiology

Topics
Publications

Scharf DH, Dworschak JD, Chankhamjon P, Scherlach K, Heinekamp T, Brakhage AA, Hertweck C (2018) Reconstitution of Enzymatic Carbon-Sulfur Bond Formation Reveals Detoxification-Like Strategy in Fungal Toxin Biosynthesis. ACS Chem Biol [Epub ahead of print] Details PubMed

Dunbar KL, Scharf DH, Litomska A, Hertweck C (2017) Enzymatic Carbon-Sulfur Bond Formation in Natural Product Biosynthesis. Chem Rev 117(8), 5521-5577. Details PubMed

Johns A, Scharf DH, Gsaller F, Schmidt H, Heinekamp T, Straßburger M, Oliver JD, Birch M, Beckmann N, Dobb KS, Gilsenan J, Rash B, Bignell E, Brakhage AA, Bromley MJ (2017) A Nonredundant Phosphopantetheinyl Transferase, PptA, Is a Novel Antifungal Target That Directs Secondary Metabolite, Siderophore, and Lysine Biosynthesis in Aspergillus fumigatus and Is Critical for Pathogenicity. MBio 8(4), Details PubMed

Marion A, Groll M, Scharf DH, Scherlach K, Glaser M, Sievers H, Schuster M, Hertweck C, Brakhage AA, Antes I, Huber EM (2017) Gliotoxin Biosynthesis: Structure, Mechanism, and Metal Promiscuity of Carboxypeptidase GliJ. ACS Chem Biol 12(7), 1874-1882. Details PubMed

Scharf DH, Brakhage AA, Mukherjee PK (2016) Gliotoxin- bane or boon? Environ Microbiol 18(4), 1096-1109. (Review) Details PubMed

Horn F, Habel A, Scharf DH, Dworschak J, Brakhage AA, Guthke R, Hertweck C, Linde J (2015) Draft Genome Sequence and Gene Annotation of the Entomopathogenic Fungus Verticillium hemipterigenum. Genome Announc 3(1), pii: e01439-14. Details PubMed Open Access

Kalb D, Heinekamp T, Lackner G, Scharf DH, Dahse H-M, Brakhage AA, Hoffmeister D (2015) Genetic engineering activates biosynthesis of aromatic fumaric acid amides in the human pathogen Aspergillus fumigatus. Appl Environ Microbiol 81, 1594-1600. Details PubMed

Bacher P, Kniemeyer O, Teutschbein T, Thoen M, Vödisch M, Wartenberg D, Scharf DH, Koester-Eiserfunke N, Schütte M, Dübel S, Assenmacher M, Brakhage AA, Scheffold A (2014) Identification of immunogenic antigens from Aspergillus fumigatus by direct multi-parameter characterization of specific conventional and regulatory CD4+ T cells. Journal of Immunology 197(7), 3332-3343. Details PubMed

Habel A, Scharf DH, Heinekamp T, Brakhage AA, Hertweck C (2014) Opposed Effects of Enzymatic Gliotoxin N-and S-Methylations J.Am.Chem.Soc. (136), 11674-11679. Details

Scharf DH, Groll M, Habel A, Heinekamp T, Hertweck C, Brakhage AA, Huber EM (2014) Flavoenzyme-catalyzed formation of disulfide bonds in natural products. Angew Chem Int Ed Engl 53(8), 2221-2224. Details PubMed

Scharf DH, Habel A, Heinekamp T, Brakhage AA, Hertweck C (2014) Opposed effects of enzymatic gliotoxin N- and S-methylations. J Am Chem Soc 136(33), 11674-11679. Details PubMed

Scharf DH, Heinekamp T, Brakhage AA (2014) Human and plant fungal pathogens: the role of secondary metabolites. PLOS Pathog 10(1), e1003859. (Review) Details PubMed

Knop K, Pavlov GM, Rudolph T, Martin K, Pretzel D, Jahn BO, Scharf DH, Brakhage AA, Makarov V, Möllmann U, Schacher FH, Schubert US (2013) Amphiphilic star-shaped block copolymers as unimolecular drug delivery systems: investigations using a novel fungicide Soft Matter 3(9), 715-726. Details

Knop K, Pretzel D, Urbanek A, Rudolph T, Scharf DH, Schallon A, Wagner M, Schubert S, Kiehntopf M, Brakhage AA, Schacher FH, Schubert US (2013) Star-shaped drug carriers for doxorubicin with POEGMA and POEtOxMA brush-like shells: a structural, physical, and biological comparison. Biomacromolecules 14(8), 2536-2548. Details PubMed

Scharf DH, Brakhage AA (2013) Engineering fungal secondary metabolism: a roadmap to novel compounds. J Biotechnol 163(2), 179-183. (Review) Details PubMed

Scharf DH, Chankhamjon P, Scherlach K, Heinekamp T, Willing K, Brakhage AA, Hertweck C (2013) Epidithiodiketopiperazine biosynthesis: a four-enzyme cascade converts glutathione conjugates into transannular disulfide bridges. Angew Chem Int Ed Engl 52(42), 11092-11095. Details PubMed

Huber EM, Scharf DH, Hortschansky P, Groll M, Brakhage AA (2012) DNA minor groove sensing and widening by the CCAAT-binding complex. Structure 20(10), 1757-1768. Details PubMed

Scharf DH, Chankhamjon P, Scherlach K, Heinekamp T, Roth M, Brakhage AA, Hertweck C (2012) Epidithiol formation by an unprecedented twin carbon-sulfur lyase in the gliotoxin pathway. Angew Chem Int Ed Engl 51(40), 10064-10068. Details PubMed

Scharf DH, Heinekamp T, Remme N, Hortschansky P, Brakhage AA, Hertweck C (2012) Biosynthesis and function of gliotoxin in Aspergillus fumigatus. Appl Microbiol Biotechnol 93(2), 467-472. Details PubMed

Scharf DH, Remme N, Habel A, Chankhamjon P, Scherlach K, Heinekamp T, Hortschansky P, Brakhage AA, Hertweck C (2011) A dedicated glutathione S-transferase mediates carbon-sulfur bond formation in gliotoxin biosynthesis. J Am Chem Soc 133(32), 12322-12325. Details PubMed

Scharf DH, Remme N, Heinekamp T, Hortschansky P, Brakhage AA, Hertweck C (2010) Transannular disulfide formation in gliotoxin biosynthesis and its role in self-resistance of the human pathogen Aspergillus fumigatus. J Am Chem Soc 132(29), 10136-10141. Details PubMed

Thön M, Al Abdallah Q, Hortschansky P, Scharf DH, Eisendle M, Haas H, Brakhage AA (2010) The CCAAT-binding complex coordinates the oxidative stress response in eukaryotes. Nucleic Acids Res 38(4), 1098-1113. Details PubMed

Brakhage AA, Thön M, Spröte P, Scharf DH, Al-Abdallah Q, Wolke SM, Hortschansky P (2009) Aspects on evolution of fungal beta-lactam biosynthesis gene clusters and recruitment of trans-acting factors. Phytochemistry 70(15-16), 1801-1811. Details PubMed

Spröte P, Hynes MJ, Hortschansky P, Shelest E, Scharf DH, Wolke SM, Brakhage AA (2008) Identification of the novel penicillin biosynthesis gene aatB of Aspergillus nidulans and its putative evolutionary relationship to this fungal secondary metabolism gene cluster. Mol Microbiol 70(2), 445-461. Details PubMed