Uwe Horn

Dr. Uwe Horn

Bio Pilot Plant

Phone: +49 3641 532-1500 Fax: +49 3641 532-2500 Email: uwe.horn@leibniz-hki.de

Main Research Areas
  • Development and optimisation of biotechnological processes for natural products and proteins
  • Ultra high throughput screening of natural product producers in microfluidic systems
  • Development of technologies for the in vivo monitoring of bioprocess parameters
Curriculum vitae

Professional Career

Since 2004  Head of the Pilot Plant, Leibniz Institute for Natural Product Research and Infection Biology – Hans-Knöll-Institute (HKI) Jena 
1996-2004 Research assistant at the HKI
1994-1996 Postdoc of the working group of Prof. Dr. Andreas Plückthun, Dept. of Biochemistry, University Zurich, Schwitzerland, funded through EMBO Fellowship
1994 Dr. rer. nat. in mikrobiology, „magna cum laude“, Martin Luther University Halle-Wittenberg, Institut für Allgemeine Mikrobiologie
1989-1994 Scientific assistant at the Institut für Allgemeine Mikrobiologie at the Martin Luther University Halle-Wittenberg
1989 Diploma in biology at the Martin Luther Universiyt Halle-Wittenberg
1986-1989 Studies in biology at the Martin Luther University, Halle-Wittenberg
1984-1986 Studies in biology at the Ernst Moritz Arndt University, Greifswald

Awards · Appointments · Scientific Activities

Member of the Editorial Board of Microbial Research and reviewer of biotechnologically-oriented journals, e. g. Appl Environ Microbiol.; Appl Microbiol Biotechnol.; Microbial Cell Factories; Microbial Research.
Publications

Funk J, Schaarschmidt B, Slesiona S, Hallström T, Horn U, Brock M (2016) The glycolytic enzyme enolase represents a plasminogen-binding protein on the surface of a wide variety of medically important fungal species. Int J Med Microbiol 306(1), 59-68. Details PubMed

Garvey M, Baumann M, Wulff M, Kumar ST, Markx D, Morgado I, Knüpfer U, Horn U, Mawrin C, Fändrich M, Balbach J (2016) Molecular architecture of Aβ fibrils grown in cerebrospinal fluid solution and in a cell culture model of Aβ plaque formation. Amyloid 23(2), 76-85. Details PubMed

Horn F, Linde J, Mattern DJ, Walther G, Guthke R, Scherlach K, Martin K, Brakhage AA, Petzke L, Valiante V (2016) Draft genome sequences of fungus Aspergillus calidoustus. Genome Announc 4(2), e00102-16. Details PubMed

Kollmer M, Meinhardt K, Haupt C, Liberta F, Wulff M, Linder J, Handl L, Heinrich L, Loos C, Schmidt M, Syrovets T, Simmet T, Westermark P, Westermark GT, Horn U, Schmidt V, Walther P, Fändrich M (2016) Electron tomography reveals the fibril structure and lipid interactions in amyloid deposits. Proc Natl Acad Sci U S A 113(20), 5604-5609. Details PubMed Supplement

Lüdecke C, Roth M, Yu W, Horn U, Bossert J, Jandt KD (2016) Nanorough titanium surfaces reduce adhesion of Escherichia coli and Staphylococcus aureus via nano adhesion points Colloids Surf B Biointerfaces 145, 617-625. Details PubMed

Wulff M, Baumann M, Thümmler A, Yadav JK, Heinrich L, Knüpfer U, Schlenzig D, Schierhorn A, Rahfeld JU, Horn U, Balbach J, Demuth HU, Fändrich M (2016) Enhanced Fibril Fragmentation of N-Terminally Truncated and Pyroglutamyl-Modified Aβ Peptides. Angew Chem Int Ed 55(16), 5081-5084. Details PubMed

Schwenk D, Nett M, Dahse H-M, Horn U, Blanchette RA, Hoffmeister D (2014) Injury-induced biosynthesis of methyl-branched polyene pigments in a white-rotting basidiomycete J Nat Prod 77, 2658-2663. Details PubMed

Wacker J, Rönicke R, Westermann M, Wulff M, Reymann KG, Dobson CM, Horn U, Crowther DC, Luheshi LM, Fändrich M (2014) Oligomer-targeting with a conformational antibody fragment promotes toxicity in Aβ-expressing flies. Acta Neuropathol Commun 2:43, Details PubMed Open Access PDF

Biermann M, Bardl B, Vollstädt S, Linnemann J, Knüpfer U, Seidel G, Horn U (2013) Simultaneous analysis of the non-canonical amino acids norleucine and norvaline in biopharmaceutical-related fermentation processes by a new ultra-high performance liquid chromatography approach. Amino Acids 44(4), 1225-1231. Details PubMed Open Access PDF

Biermann M, Linnemann J, Knüpfer U, Vollstädt S, Bardl B, Seidel G, Horn U (2013) Trace element associated reduction of norleucine and norvaline accumulation during oxygen limitation in a recombinant Escherichia coli fermentation. Microb Cell Fact 12, 116. Details PubMed Open Access PDF

Morgado I, Wieligmann K, Bereza M, Rönicke R, Meinhardt K, Annamalai K, Baumann M, Wacker J, Hortschansky P, Malešević M, Parthier C, Mawrin C, Schiene-Fischer C, Reymann KG, Stubbs MT, Balbach J, Görlach M, Horn U, Fändrich M (2012) Molecular basis of β-amyloid oligomer recognition with a conformational antibody fragment. Proc Natl Acad Sci U S A 109(31), 12503-12508. Details PubMed Open Access PDF

Sarkar A, Funk AN, Scherlach K, Horn F, Schroeckh V, Chankhamjon P, Westermann M, Roth M, Brakhage AA, Hertweck C, Horn U (2012) Differential expression of silent polyketide biosynthesis gene clusters in chemostat cultures of Aspergillus nidulans. J Biotechnol 160(1-2), 64-71. Details PubMed

Garvey M, Tepper K, Haupt C, Knüpfer U, Klement K, Meinhardt J, Horn U, Balbach J, Fändrich M (2011) Phosphate and HEPES buffers potently affect the fibrillation and oligomerization mechanism of Alzheimer's Aβ peptide. Biochem Biophys Res Commun 409(3), 385-388. Details PubMed

Haupt C, Bereza M, Kumar ST, Kieninger B, Morgado I, Hortschansky P, Fritz G, Röcken C, Horn U, Fändrich M (2011) Pattern recognition with a fibril-specific antibody fragment reveals the surface variability of natural amyloid fibrils. J Mol Biol 408(3), 529-540. Details PubMed

Haupt C, Morgado I, Kumar ST, Parthier C, Bereza M, Hortschansky P, Stubbs MT, Horn U, Fändrich M (2011) Amyloid fibril recognition with the conformational B10 antibody fragment depends on electrostatic interactions. J Mol Biol 405(2), 341-348. Details PubMed

Scherlach K, Sarkar A, Schroeckh V, Dahse HM, Roth M, Brakhage AA, Horn U, Hertweck C (2011) Two induced fungal polyketide pathways converge into antiproliferative spiroanthrones. Chembiochem 12(12), 1836-1839. Details PubMed

Schild L, Heyken A, de Groot PW, Hiller E, Mock M, de Koster C, Horn U, Rupp S, Hube B (2011) Proteolytic cleavage of covalently linked cell wall proteins by Candida albicans Sap9 and Sap10. Eukaryot Cell 10(1), 98-9109. Details PubMed Open Access PDF

Bergmann S, Funk AN, Scherlach K, Schroeckh V, Shelest E, Horn U, Hertweck C, Brakhage AA (2010) Activation of a silent fungal polyketide biosynthesis pathway through regulatory cross talk with a cryptic nonribosomal peptide synthetase gene cluster. Appl Environ Microbiol 76(24), 8143-8149. Details PubMed Open Access PDF Supplement

Schachtschabel D, Menzel KD, Krauter G, David A, Roth M, Horn U, Boland W, Wöstemeyer J, Schimek C (2010) Production and derivate composition of trisporoids in extended fermentation of Blakeslea trispora. Appl Microbiol Biotechnol 88(1), 241-249. Details PubMed

Siurkus J, Panula-Perälä J, Horn U, Kraft M, Rimseliene R, Neubauer P (2010) Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures. Microb Cell Fact 9, 35. Details PubMed Open Access

Kawe M, Horn U, Plückthun A (2009) Facile promoter deletion in Escherichia coli in response to leaky expression of very robust and benign proteins from common expression vectors. Microb Cell Fact 8, 8. Details PubMed Open Access PDF

Borowski A, Kuepper M, Horn U, Knüpfer U, Zissel G, Höhne K, Luttmann W, Krause S, Virchow JC, Friedrich K (2008) Interleukin-13 acts as an apoptotic effector on lung epithelial cells and induces pro-fibrotic gene expression in lung fibroblasts. Clin Exp Allergy 38(4), 619-628. Details PubMed

Habicht G, Haupt C, Friedrich RP, Hortschansky P, Sachse C, Meinhardt J, Wieligmann K, Gellermann GP, Brodhun M, Götz J, Halbhuber KJ, Röcken C, Horn U, Fändrich M (2007) Directed selection of a conformational antibody domain that prevents mature amyloid fibril formation by stabilizing Abeta protofibrils. Proc Natl Acad Sci U S A 104(49), 19232-19237. Details PubMed

Kraft M, Knüpfer U, Wenderoth R, Kacholdt A, Pietschmann P, Hock B, Horn U (2007) A dual expression platform to optimize the soluble production of heterologous proteins in the periplasm of Escherichia coli. Appl Microbiol Biotechnol 76(6), 1413-1422. Details PubMed

Kraft M, Knüpfer U, Wenderoth R, Pietschmann P, Hock B, Horn U (2007) An online monitoring system based on a synthetic sigma32-dependent tandem promoter for visualization of insoluble proteins in the cytoplasm of Escherichia coli. Appl Microbiol Biotechnol 75(2), 397-406. Details PubMed

Kraft M, Radke D, Wieland GD, Zipfel PF, Horn U (2007) A fluorogenic substrate as quantitative in vivo reporter to determine protein expression and folding of tobacco etch virus protease in Escherichia coli. Protein Expr Purif 52(2), 478-484. Details PubMed