Butyrate modulates antioxidant enzyme expression in malignant and non-malignant human colon tissues.

Jahns F, Wilhelm A, Jablonowski N, Mothes H, Greulich KO, Glei M (2014) Butyrate modulates antioxidant enzyme expression in malignant and non-malignant human colon tissues. Mol Carcinog [Epub ahead of print]

Abstract

The induction of antioxidant enzymes is an important mechanism in colon cancer chemoprevention, but the response of human colon tissue to butyrate, a gut fermentation product derived from dietary fiber, remains largely unknown. Therefore, our study investigated the effect of a butyrate treatment on catalase (CAT) and superoxide dismutase (SOD2) in matched human colon tissues of different transformation stages (n = 3-15 in each group) ex vivo. By performing quantitative real-time PCR, Western blot, and spectrophotometric measurements, we found an increase in SOD2 at expression and activity level in colonic adenocarcinomas (mRNA: 1.96-fold; protein: 1.41-fold, activity: 1.8-fold; P 

Leibniz-HKI-Authors

Nadja Jablonowski

Identifier

doi: 10.1002/mc.22102

PMID: 24677319