Scalable hybrid synthetic/biocatalytic route to psilocybin.

Fricke J, Kargbo R, Regestein L, Lenz C, Peschel G, Rosenbaum MA, Sherwood A, Hoffmeister D (2020) Scalable hybrid synthetic/biocatalytic route to psilocybin. Chem Eur J 37(26), 8281-8285.


Psilocybin, the principal indole alkaloid of Psilocybe mushrooms, is currently undergoing clinical trials as a medication against treatment-resistant depression and major depressive disorder. The psilocybin supply for pharmaceutical purposes is met by synthetic chemistry. We replaced the problematic phosphorylation step during synthesis with the mushroom kinase PsiK. This enzyme was biochemically characterized and used to produce one gram of psilocybin from psilocin within 20 minutes. We also describe a pilot-scale protocol for recombinant PsiK that yielded 150 mg enzyme in active and soluble form. Our work consolidates the simplicity of tryptamine chemistry with the specificity and selectivity of enzymatic catalysis and helps provide access to an important drug at potentially reasonable cost.


Miriam Agler-Rosenbaum
Janis Fricke
Dirk Hoffmeister
Claudius Lenz
Gundela Peschel
Lars Regestein


doi: 10.1002/chem.202000134

PMID: 32101345