In this chapter, we explore the wide range of functions RNA plays in mediating interactions between fungi and their hosts in the animal kingdom. We cover examples of RNA in the regulation of gene expression, novel RNA structures and non-coding RNAs with links to fungal biology, as well as the emerging fields of epimutation and extracellular RNA. Next, the host response to fungal infection is investigated by considering cases of RNA recognition by host pattern recognition receptors, together with host regulatory mechanisms dependent on non-coding RNAs. Many of these themes are then revisited in the discussion of interkingdom communication, where RNA is sent between fungi and animals to influence the outcome of a given infection. We end by discussing the recent progress in RNA-based therapeutics against human fungal pathogens, where this foundation of basic research is leading to potential improvements in the way we treat human diseases.