Functionally distinct modules operate two consecutive alpha,beta-->beta,gamma double-bond shifts in the rhizoxin polyketide assembly line.
Abstract
Shift work: Biochemical analysis of the rhizoxin pathway revealed that the diene moiety is not shifted all at once, but through distinct enzymatic operations. The first shift occurs by a formal β,γ-dehydration in module 7, while the second double bond is first generated by module 8 and then shifted by an unprecedented “shift module” with a novel type of DH* domain (see scheme). ACP=acyl carrier protein, DH*=dehydratase-like shift domain.
Identifier
doi: 10.1002/anie.200905467
PMID: 20033973