A Gram-positive-staining, aerobic, endospore-forming bacterium, isolated as a contamination from an enrichment of enteric bacteria from surface water, was studied using a polyphasic taxonomic approach. 16S rRNA gene sequence similarity comparisons revealed that strain FSt3A(T) was grouped in the genus Lysinibacillus, most closely related to Lysinibacillus xylanilyticus XDB9(T) (98.1%), Lysinibacillus parviboronicapiens BAM-582(T) and Lysinibacillus sphaericus DSM 28(T) (both 98.0%). The 16S rRNA gene sequence similarity to other species of the genus Lysinibacillus was