Epidithiodiketopiperazine biosynthesis: a four-enzyme cascade converts glutathione conjugates into transannular disulfide bridges.

Scharf DH, Chankhamjon P, Scherlach K, Heinekamp T, Willing K, Brakhage AA, Hertweck C (2013) Epidithiodiketopiperazine biosynthesis: a four-enzyme cascade converts glutathione conjugates into transannular disulfide bridges. Angew Chem Int Ed Engl 52(42), 11092-11095. PubMed

Abstract

Enzyme quartet: Isolation of the first sulfur-bearing intermediate of the gliotoxin pathway in Aspergillus fumigatus and successful in vitro conversion of the bisglutathione adduct into an intact epidithiodiketopiperazine by a four-enzyme cascade (including glutamyltransferase GliK and dipeptidase GliJ) revealed an outstanding adaptation of a primary metabolic pathway into natural product biosynthesis that is widespread in fungi.

Involved Units and Groups

Biomolecular Chemistry Molecular and Applied Microbiology Bio Pilot Plant

HKI-Authors

Prof. Dr. Axel A. Brakhage Prof. Dr. Christian Hertweck Dr. Thorsten Heinekamp Dr. Daniel H. Scharf Dr. Pranatchareeya Chankhamjon Karsten Willing

Identifier

doi: 10.1002/anie.201305059 PMID: 24039048