We investigate, develop and optimize biotechnological processes

The research of the HKI Bio Pilot Plant is mainly focused on the development and optimization of biotechnological processes of natural product producers, including strain development and optimization. In the German academic sector, the Bio Pilot Plant offers unique opportunities for the cultivation of a wide spectrum of microorganisms (bacteria, lower and higher fungi, genetically modified organisms, safety group 1 and 2) from picoliter droplets, via microtiter plates and shake flask cultures to laboratory and pilot scale bioreactors of up to 4.2 m³. All necessary analytical and purification processes including the appropriate technical equipment are available for high quality purifications of natural products, proteins and biopolymers. We are well experienced in the cultivation of microorganisms in the chemostat, anaerobic fermentation conditions, and fermentation at high cell densities. The establishment of a microfluidic platform for ultra-high-throughput-screening of natural products and enzymes and the integration with bioelectrochemistry are examples for the development of innovative technologies. The Bio Pilot Plant closely co-operates with the research departments of the HKI and groups of the Friedrich Schiller University and the Ernst Abbe University of Applied Sciences, Jena.


Miriam Agler-Rosenbaum
Head of department
Lars Regestein
Deputy head of department


Harnisch F, Deutzmann JS, Boto ST, Rosenbaum MA (2024) Microbial electrosynthesis: opportunities for microbial pure cultures. Trends Biotechnol S0167-7799(24), 00033-7. (Review)
Schlembach I, Bardl B, Regestein L, Rosenbaum MA (2024) Nonengineered fungus provides a shortcut from cellulose to bulk Erythro-isocitric acid. ACS Sustainable Chem Eng 12(9), 3408-3418.
Tiso T, Demling P, Karmainski T, Oraby A, Eiken J, Liu L, Bongartz P, Wessling M, Desmond P, Schmitz S, Weiser S, Emde F, Czech H, Merz J, Zibek S, Blank LM, Regestein L (2024) Foam control in biotechnological processes—challenges and opportunities. Discov Chem Eng 4(2),